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Original Research Article | OPEN ACCESS

Thymoquinone enhances sperm DNA integrity in nicotine-induced infertile male rats

Farah Dayana Rosli1, Noor Hashida Hashim2 , Yusmin Mohd Yusuf3, Khairul Osman4, Siti Fatimah Ibrahim5, Nurul Kabir6

1Institute of Graduate Studies; 2Biology Division, Center for Foundation Studies in Science; 3Centre for Research in Biotechnology for Agriculture, University of Malaya, 50603; 4Faculty of Allied Health Sciences, National University of Malaysia, 50586; 5Faculty of Medicine, National University of Malaysia, 56000; 6Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.

For correspondence:-  Noor Hashim   Email: nhhpasum@um.edu.my   Tel:+60379674629

Accepted: 22 May 2019        Published: 30 June 2019

Citation: Rosli FD, Hashim NH, Yusuf YM, Osman K, Ibrahim SF, Kabir N. Thymoquinone enhances sperm DNA integrity in nicotine-induced infertile male rats. Trop J Pharm Res 2019; 18(6):1219-1225 doi: 10.4314/tjpr.v18i6.11

© 2019 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To assess the effects of thymoquinone (TQ) on the integrity of sperm DNA in nicotine-induced sperm impairment in rats.
Methods: Adult male Sprague Dawley rats were randomized into four equal groups: control group received normal saline orally for 60 days; nicotine group was subcutaneously injected with 5 mg/kg/day nicotine for 30 days and then given normal saline for the next 30 days; TQ group was given normal saline for 30 days followed by TQ at 5 mg/kg/day for 30 days; and nicotine-TQ group received 5 mg/kg of nicotine for 30 days and 5mg/kg of TQ for another 30 days. Sperm DNA breakages were evaluated using Comet assay. The expression levels of protamine 1 (PT1) and transition nuclear protein 2 (Tnp2) genes which are essential for the proper compaction of the sperm DNA were analyzed by quantitative polymerase chain reaction (qPCR).
Results: Thymoquinone significantly decreased DNA fragmentation in the sperm of nicotine-treated rats. However, there was no change in PT1 gene expression. Tnp2 was downregulated in the nicotine group and slightly upregulated in nicotine-TQ group (p < 0.05).
Conclusion: The results demonstrate the potential benefits of TQ in improving sperm DNA quality of nicotine-induced male infertility.

Keywords: Thymoquinone, Infertility, Nicotine, Sperm, DNA integrity, Protamine 1, Transition nuclear protein 2

Impact Factor
Thompson Reuters (ISI): 0.523 (2021)
H-5 index (Google Scholar): 39 (2021)

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